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KMID : 1039420180520050275
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Journal of Pathology and Translational Medicine
2018 Volume.52 No. 5 p.275 ~ p.282
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Differential MicroRNA Expression between EGFR T790M and L858R Mutated Lung Cancer
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Kim Ji-Yeon
Lee Woo-Jeong
Park Ha-Young
Kim Ah-Rong
Shin Dong-Hoon
Lee Chang-Hun
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Abstract
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Background: MicroRNAs (miRNAs) are short, non-coding RNAs that mediate post-transcriptional gene regulation. They are commonly deregulated in human malignancies, including non-small cell lung cancer (NSCLC). The aim of this study is to investigate miRNA expression in T790M-mutated NSCLC resistant to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors.
Methods: Six cases of resected NSCLC harboring the T790M mutation were examined. We performed miRNA time polymerase chain reaction (PCR) array profiling using EGFR T790M-mutated NSCLC and L858R-mutated NSCLC. Once identified, miRNAs that were differentially expressed between the two groups were validated by quantitative real-time polymerase chain reaction (qRT-PCR).
Results: miRNA PCR array profiling revealed three up-regulated miRNAs whose expression levels were altered 4.0-fold or more in the EGFR T790M mutation group than in the L858R group: miR-1 (fold change, 4.384), miR-196a (fold change, 4.138), and miR-124 (fold change, 4.132). The three differentially expressed miRNAs were validated by qRT-PCR, and they were found to be overexpressed in the T790M group relative to L858R group. In particular, expression levels of miR-1 and miR-124 were significantly higher in the T790M group (p-value of miR-1 = .004, miR-124 = .007, miR-196a = .096).
Conclusions: MiR-1, miR-124, and miR-196a are overexpressed in EGFR T790M mutated NSCLC.
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KEYWORD
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MicroRNAs, Lung neoplasms, EGFR T790M mutation
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